Formulation and Testing of Antioxidant Activity of Lotion Made from Purified Tomato Fruit Extract (Solanum lycopersicum L.) with Various Cetyl Alcohol Concentrations

Abstract

Tomatoes contain bioactive compounds such as flavonoids, vitamin C, vitamin E, and carotenoids, with lycopene as the highest antioxidant. This study aimed to formulate a purified tomato extract into a lotion preparation and to determine its physical stability and antioxidant activity with varying amounts of cetyl alcohol. The purified tomato extract was obtained by adding methanol as an antisolvent. After separating the pulp and water, the pulp was macerated (1:4), and the water was subjected to a liquid-liquid extraction (1:2) with n-hexane. The extract was evaporated until a thick extract was obtained, precipitated with methanol, and then filtered. Carr-Price reagent was used to identify lycopene. The purified tomato extract was formulated into a lotion with a concentration of 3% and varying amounts of cetyl alcohol (2.5%, 5%, and 7.5%), which served as a stiffening agent). Physical stability was evaluated, and antioxidant activity was tested using the DPPH method. Data were analyzed using SPSS. The results showed that purified tomato fruit extract lotion with cetyl alcohol variations in formulas 3, 4, and 5 had good physical quality and decreased stability after storage. The antioxidant activity in formulas 3, 4, and 5 was classified as strong, namely 92.96 ppm; 95.28 ppm; and 98.51 ppm, respectively. Therefore, considering the variation in cetyl alcohol, formula 4 was the best formula by maintaining the viscosity of the lotion preparation and having strong antioxidant activity.